VIB-VUB facility for Bio Electron Cryogenic Microscopy, Brussels
If you are interested in practical aspects of working with a JEOL CryoARM 300, then this is the paper for you.
https://journals.iucr.org/m/issues/2020/04/00/pw5013/index.html
Scripts used can be accessed via Github: https://github.com/BECM-VIB?tab=repositories
On the 12th and 13th of December we held our first CryoEM school in Brussels. The aim was to give the basic knowledge of what CryoEM is and what it can do for you. We had theoretical lectures ranging from “What is EM?” to “Basics of data processing and image formation”. And it was accompanied by practical Demonstrations of Instrumentation and processing software and some case studies showing real life struggle and success.
Please welcome Adam Schroefel as our newest addition to the facility. As an expert microscopist he will support the users in data collection and microscope maintenance. With his extensive background in Cryo Tomography he will also involved in the further setup of a tomography workflow on our CryoARM.
A proper storage system is the first step to get beautiful EM images. So instead of a bunch of tubes and way too many ropes we are now using the Subangstrom system .
So your grids are well taken care of.
Finally it runs. Now with SerialEM we can collect up to 90 images per hour with 40 frames per image on K2 in counting mode. Many thanks to Jaap Brink and David Mastronade for their help with setting up and troubleshooting SerialEM. The microscope is very stable and the benchmarking is under way. The first data have been collected from GroEL .
