Job opening Staff scientist at BECM

The VIB Center for Structural Biology (CSB) in Brussels seeks to recruit a Cryo-electron Microscopy (cryo-EM) scientist to support and develop single-particle and tomography projects.

The research scientist will be appointed to the VIB-VUB facility for Bio Electron Cryogenic Microscopy (BECM, www.becm.be), which serves as technology platform for CSB.

BECM has a state-of-the-art facility for cryo-electron microscopy and houses a JEOL cryoARM300 equipped with in-column energy filter, phase plate and K3 detector and is complemented by two screening JEOL JEM-1400 120 kV microscopes. The facility was established in 2018 and serves a large community of users including users from VIB, Belgium and Europe from academia and industry.

Details may be found here:

https://jobso.id/d6ue

Key responsibilities:

  • Support a range of cryo-EM projects through interaction with CSB and external scientists
  • Contribute to maintenance of the microscopes
  • Contribute to the development of single particle cryo-EM and/or implementation and development for state-of-the-art tomography
  • Provide user training in experimental and computational aspects of cryo-EM

About us:

VIB Center for Structural Biology houses research groups with strong expertise in protein biochemistry, X-ray crystallography, NMR, biophysics, nanobody technology and electron microscopy.  Our department tightly collaborates with other VIB departments (www.vib.be) on cutting edge biological research problems ranging from human diseases to plant biotechnology.

Key Experience and competences

  • Master degree in Natural Sciences or higher
  • At least two years of work experience in electron microscopy
  • Basic knowledge of Linux and programing skills
  • Good communication skills in English

Desirable

  • experience is structural biology
  • Experience in single particle or cryo-tomography data collection and analysis
  • Flexible working practice
  • Ability to troubleshoot
  • Experience with multi-user facilities

Deadline until a suitable candidate is found.

For further enquiries please contact Marcus Fislage (marcus.fislage@vub.vib.be)

Successful finish of our first CryoEM school

On the 12th and 13th of December we held our first CryoEM school in Brussels. The aim was to give the basic knowledge of what CryoEM is and what it can do for you. We had theoretical lectures ranging from “What is EM?” to “Basics of data processing and image formation”. And it was accompanied by practical Demonstrations of Instrumentation and processing software and some case studies showing real life struggle and success.

These 35 attendees from around Belgium are now equipped with theoretical and practical knowledge to use CryoEM successfully in their projects

Welcome Adam as new microscopist at BECM

Please welcome Adam Schroefel as our newest addition to the facility. As an expert microscopist he will support the users in data collection and microscope maintenance. With his extensive background in Cryo Tomography he will also involved in the further setup of a tomography workflow on our CryoARM.

First benchmarking

Finally it runs. Now with SerialEM we can collect up to 90 images per hour with 40 frames per image on K2 in counting mode. Many thanks to Jaap Brink and David Mastronade for their help with setting up and troubleshooting SerialEM. The microscope is very stable and the benchmarking is under way. The first data have been collected from GroEL .

EM images taken with GroEL sample leads to a reconstruction reaching 2.8 A resolution.
With 1.2 Å/pix the average resolution of reconstruction is 2.8 Å with  local resolution down to 2.7 Å. 
The obtained electron density is in agreement with the stated resolution.